PMID: PMC4256492-1-6

 

    Legend: Gene, Sites

Title : Comparison of the Lubricin O-glycomap with Predicted O-glycosylation Sites

Abstract :
  1. The glycosylations identified here were compared with currently available O-glycosylation prediction tools
  2. The glycosylation prediction tool NetOGlyc4.0 (39) is based on in vivo identified O-glycosylation sites and predicted almost double the number of O-glycosylation sites identified here (almost 90% of all Ser/Thr residues ) (Fig. 4C)
  3. In addition to the STP-rich region , most of the Ser/Thr residues in the N-terminal region were predicted to be O-glycosylated (Fig. 4C)
  4. An individual ppGalNAc T in vitro enzymatic specificity–based prediction tool, ISOGlyP (40), predicted that 51% (191) of the sites were glycosylated (Figs. 4C and 4D) utilizing all ppGalNAc T specificities available in the tool
  5. This prediction is closer to the 168 detected in this study (Fig. 4B)
  6. ISOGlyP predicted that a high proportion of the Thr in the STP-rich region would be glycosylated, as was also shown by the MS analysis (Table I, supplemental Fig
  7. S2, supplemental Table S1)
  8. However, no single ppGalNAc T of those included in the tool was able to glycosylate all the sites identified via MS. The ubiquitously expressed GALNT1 and -2 were suggested to glycosylate only 76% of the total sites found via MS (Fig. 4D)
  9. In total, 166 out of 191 (87%) sites predicted by the nine available genes in the software were identified through MS analysis (Fig. 4D)
  10. This suggested that a GALNT not included in ISOGlyP might be responsible for at least some of the glycosylation on synovial lubricin (Fig. 4D and supplemental Table S2)
Output (sent_index, trigger, protein, sugar, site):
  • 10. glycosylation, , lubricin, -, -
  • 2. O-glycosylation, , -, -, residues
  • 2. O-glycosylation, , -, -, sites
  • 2. identified, , -, -, residues
  • 2. identified, , -, -, sites
  • 3. O-glycosylated, , -, -, residues in
  • 4. glycosylated, , -, -, sites
  • 8. glycosylate, , -, -, sites
Output(Part-Of) (sent_index, protein, site):
  • 3. STP, region
  • 6. STP, region
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX