PMID: PMC4256492-1-7

 

    Legend: Gene, Sites

Title : Investigating the Expression of Glycosyltransferase Genes and Glycosylation

Abstract :
  1. An alternate method for understanding glycosylation is to investigate the Golgi apparatus glycosyltransferases responsible for glycosylating lubricin
  2. Because the ISOGlyP results suggested less common transferases were necessary for lubricin glycosylation, the expression of ppGalNAc Ts from human primary FLSs isolated from RA and OA patients was investigated
  3. These types of cell lines are known to produce lubricin ( 1 )
  4. The relative quantifications of all transcripts were normalized against β-actin expression
  5. The average (n = 4, except for GALNT8 , where n = 3) expression of the GALNT genes is arranged in descending order of expression in Fig. 4D
  6. High mRNA expression was observed for GALNT1 , -2, -5, and 15, and lower expression was noted for the GALNT8 , -10, -12, and 16 genes
  7. The high expression of GALNT1 and -2 was in agreement with the suggestion that these two genes are ubiquitously expressed
  8. In contrast, GALNT5 and GALNT15 have been shown to display restricted expression profiles, suggesting these isoforms serve unique functions in the tissue where they are expressed (2 1 )
  9. The high expression of GALNT5 in FLSs indicated a potential role of this gene, and its relevance is increasing, as the expression of this gene has also been shown in chondrocytes (neXtProt)
  10. The GALNT5 gene was also able to correctly predict 54% of the sites identified via MS (Fig. 4D), which also indicates potential involvement of this gene in lubricin glycosylation
  11. The data showed that the highest expression was of the GALNT15 gene in the FLS cultures
  12. The specificity of this enzyme toward mucin-type domains is not currently understood (4 1 ), making its further investigation essential, especially as the gene has been shown to be one of the most expressed genes in chondrocytes and bone (2 1 , 42)
Output (sent_index, trigger, protein, sugar, site):
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX