PMID: PMC4256492-1-fig1

 

    Legend: Gene, Sites

Title : Accessibility and characterization of the glycosylated region of lubricin

Abstract :

1. A, the enriched synovial lubricin samples, before and after trypsin digestion, were separated on a 3–8% Tris acetate gel, blotted onto PVDF membrane, and then probed with lubricin-specific antibody (mouse anti- lubricin ) and carbohydrate-specific biotinylated lectins PNA, specific for core 1 (Galβ1–3GalNAc) O-glycan, and WGA, specific for sialic acid and terminal GlcNAc
2. B, SDS- PAGE (3–8 % Tris acetate gel) of the acidic glycoprotein fractions of the SF before (−) and after (+) partial de-glycosylation stained with Coomassie Brilliant Blue
3. C, SF lubricin was in-solution digested, and non-modified peptides were identified via mass spectrometry for protein coverage determination (black)
4. The low protein coverage (in particular the mucin domain ) suggests that the mucin domain is extensively glycosylated
5. Some of the core 1 structures were removed by partial de-glycosylation, and the previously glycosylated peptides were identified for protein coverage (gray)
6. The results suggest that lubricin contains an extended STP-rich region relative to the mucin domain previously defined by UniProt

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylated, , -, -, region
• 2. glycoprotein, , glycoprotein, -, -
• 4. glycosylated, , -, -, domain
• 5. glycosylated, , -, -, peptides

Output(Part-Of) (sent_index, protein, site):

• 0. lubricin, region
• 6. STP, region

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX