PMID: PMC4256492-1-fig2

 

    Legend: Gene, Sites

Title : Identification of lubricin mucin glycopeptides using CID

Abstract :

1. The figure shows the different glycoforms of the same tryptic peptide within the STP-rich region
2. A, CID-MS2 spectrum of the tandem repeat (EPAPTTPK) containing core 1 glycan (Galβ1–3GalNAcα1-) of the [M+2H]2+ ions at m/z 603.3
3. B, CID-MS2 spectrum of the same tandem repeat containing sialyl T antigen (NeuAcα2–3Galβ1–3GalNAcα1-) of the [M+2H]2+ ions at m/z 748.8
4. C, CID-MS2 spectrum of the same tandem repeat (EPAPTTPK) containing two separate core 1 glycans (Galβ1–3GalNAcα1-) on two adjacent threonines of the [M+2H]2+ ions at m/z 785.8
5. D, CID-MS2 spectrum of the same tandem repeat containing core 2 glycan Galβ1–3(Galβ1–4GlcNAcβ1–6)GalNAcα1- of the [M+2H]2+ ions at m/z 785.8
6. This shows that the same peptide (A, B, and D) can be differently glycosylated, indicating the complexity associated with lubricin O-glycosylation , and that CID was able to provide information about the nature of glycans and peptides
7. In the figure, yellow squares and circles represent N-acetylgalactosamine and galactose, respectively, and blue squares and pink diamonds represent N-acetylglucosamine and N-acetylneuramininc acid (sialic acid) , respectively

Output (sent_index, trigger, protein, sugar, site):

• 0. glycopeptides, , -, -, glycopeptides
• 1. glycoforms, , -, -, peptide
• 6. glycosylated, , -, -, peptide

Output(Part-Of) (sent_index, protein, site):

• 1. STP, region

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX