Title : The A, the glycosylated
peptides identified in the
STP-rich
region and non-glycosylated
peptides (light gray) in the end
domains of the
lubricin sequence before (black) and after (dark gray) partial de-glycosylation using CID and
ETD fragmentation
Abstract :
- B, graphic representation of all of the identified glycans and their positions in the protein sequence
- The figure includes the identified glycopeptide containing GalNAcα1- (dark orange, 130), core 1 Galβ1–3GalNAcα1- (chocolate, 161), core 2 Galβ1–3(Galβ1–4GlcNAcβ1–6)GalNAcα1- (blue, 85), and sialylated core 1 and 2 (pink, 73) glycans with their positions in the protein sequence
- The graph also includes the total number of identified glycosylation sites (168 sites ) in the protein sequence (black)
- The total number of sites is based on the identified peptides and the fact that two threonines in the imperfect tandem repeat variants (e.g. EPAPTTPK, SAPTTPK, EPAPTTTK) can be glycosylated
- It indicates a high number of glycosylations in the STP-rich region , whereas the N- and C-terminal regions are scarcely glycosylated
- C, the combined CID and ETD identified glycopeptides in the STP-rich region (black and dark gray) and non-glycosylated peptides (light gray) in the end domains of lubricin before (black) and after partial de-glycosylation (dark gray)
- The software used (NetOGlyc4.0 and ISOGlyP) predicted sites of lubricin O-glycosylated Ser/Thr in the protein sequence (yellow) and the total number of potential Ser/Thr sites (370)
- The results indicate that the gene-based ISOGlyP software predicted a similar number of glycosylation sites (191 sites ) as identified by the data presented in this report (168 sites )
- D, the GALNT profiling expression analysis of the 20 known human GALNT genes (top) in primary FLSs and the ISOGlyP-predicted sites by the available GALNT genes identified by the data presented in this report (bottom)
- The genes were arranged in descending order of their expression
- RQ, relative quantification
- Asterisk denotes genes not included in the ISOGlyP software
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycosylated, , -, -, peptides
- 0. non-glycosylated, , -, -, peptides
- 2. glycopeptide, , -, -, glycopeptide
- 3. glycosylation, , -, -, sites
- 4. glycosylated, , -, -, threonines
- 5. glycosylated, , -, -, regions
- 5. glycosylations, , -, -, region
- 6. glycopeptides, , -, -, glycopeptides
- 6. non-glycosylated, , -, -, peptides
- 7. O-glycosylated, , -, -, Ser/Thr
- 8. glycosylation, , -, -, sites
- 8. presented, , -, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 0. STP, region
- 0. lubricin, sequence
- 4. variants, threonines
- 5. STP, region
- 6. STP, region
- 6. lubricin, domains
- 7. -, Ser/Thr
- 7. -, sites
- 7. lubricin, Ser/Thr
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):