Title : The implication of the type and distribution of A, the predicted isoelectric point (pI) of full-length (black) and
STP-rich
regions (dark gray) for varying numbers of sialic acids identified, indicating that the pI decreases as the number of sialic acids increases
Abstract :
- B, enriched acidic SF fractions, before and after sialidase treatment, were separated on pH 3 –10 IPG gels and blotted to PVDF membrane
- Western blot analysis using lubricin-specific antibody (mAb13) indicated a pH range of synovial lubricin before sialidase treatment ( pH 4 –7.5) and a drastic increase in pH (pH 7.5) after sialidase treatment
- C, the pI of the N- (9.45–9.6) and C-terminal regions (9.69–9.98) (light gray), where very few glycosylation sites were identified, and the extended STP-rich region , which contained the majority of the protein glycosylation (4–7.5)
Output (sent_index, trigger,
protein,
sugar,
site):
- 3. glycosylation, , -, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 0. STP, regions
- 3. STP, region
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):