PMID: PMC4739677-1-15

 

    Legend: Gene, Sites

Title : Fibrinogen α and β Chain

Abstract :
  1. The blood clotting protein fibrinogen is known to be N-glycosylated at the β- and γ-chain
  2. Interestingly, a recent study by Zauner et al. could also show O-glycosylated sites and regions, seven in total, within the molecule (51)
  3. In the present study O-glycosylation of the fibrinogen alpha region aa524–528 could be confirmed; pinpointing the exact O-glycosylation site was not possible, though (supplemental Fig
  4. S5, 524GKFPG531, m/z 725.782+)
  5. Nevertheless, O-glycosylation within the fibrinogen beta region aa58–67 could be confirmed and pinpointed
  6. Here, the presence of the ETD fragment ions m/z 931.541+, 1300.541+, and 1915.501+, corresponding to z+19, c6, and c12 ions (supplemental Fig
  7. S6), 54EEAPLRPAPPPIS67, m/z 706.273+) indicates O-glycosylation at the site Ser58
  8. This contrasts recent findings by Bai et al. who reported the site Ser67 to be O-glycosylated, but not the site Ser58 (76)
  9. In agreement with previous findings, both fibrinogen O-glycopeptides (524GKFPG531, m/z 725.782+, 54EEAPLRPAPPPIS67, m/z 706.273+), detected in the present study, were found to be decorated with monosialylated T-antigens
  10. Interestingly, the peptide 54EEAPLRPAPPPIS67 was also found in its nonglycosylated form (HILIC fractions #12-#15, CID, see supplemental Table S2), which suggests only a partial occupation of the O-glycosylation site Ser58
Output (sent_index, trigger, protein, sugar, site):
  • 10. O-glycosylation, , -, -, site Ser58
  • 2. O-glycosylated, , -, -, sites
  • 3. O-glycosylation, , -, -, site
  • 7. O-glycosylation, , -, -, Ser58
  • 8. O-glycosylated, , -, -, Ser67
  • 9. O-glycopeptides, , -, -, O-glycopeptides
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 10. -, -, site Ser58
  • 7. -, -, Ser58
  • 8. -, -, Ser67

 

 

Protein NCBI ID SENTENCE INDEX