PMID: PMC4739677-1-3

 

    Legend: Gene, Sites

Title : Reproducibility of the Proteinase K Digest

Abstract :

1. Previous studies on single glycoproteins could show the successful application of Proteinase K in the context of N- and O-glycoproteomics (32, 49–52)
2. However, its application on complex samples, like human blood plasma, has not been described so far
3. Here, we have employed Proteinase K to generate (glyco) peptides from the entire (glyco)proteome of a pooled human blood plasma sample that was derived from 20 healthy donors
4. To assess the reproducibility of such a digest, five independent Proteinase K treated blood plasma samples (technical replicates) were measured with nanoRP-LC- ESI-IT-MS/MS in preliminary experiments
5. A comparison of the resulting base peak chromatograms revealed a high reproducibility of these digests, as shown in supplemental Fig
6. S1

Output (sent_index, trigger, protein, sugar, site):

• 1. glycoproteins, , glycoproteins, -, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX