PMID: PMC4739677-1-4

 

    Legend: Gene, Sites

Title : Glycopeptide Enrichment and Fractionation via HILIC-HPLC

Abstract :

1. The HILIC-HPLC fractionation carried out in the present study was optimized for the enrichment of O-glycosylated peptides (data not shown)
2. In total 17 HILIC fractions were collected and were analyzed by nanoRP-LC- ESI-IT- MS2 ( CID )
3. The acquired fragment spectra were manually screened for the presence of N- and O-glycopeptides—relying on the detection of diagnostic oxonium ions (B-ions, e.g. HexNAc1Hex1NeuAc1; m/z 657.24) and characteristic mono(oligo)-saccharide neutral loss fragment ions (Y-ions)
4. Glycopeptides were detected in five HILIC fractions (#13-#17) (Fig. 2)
5. The glycopeptides eluted in the range of 9–32 min in RP-LC-MS and clusters of glycopeptides were registered between 12–18 min, 20–22 min, and 25–29 min (exemplarily shown for fraction #15, Fig. 3)

Output (sent_index, trigger, protein, sugar, site):

• 1. O-glycosylated, , -, -, peptides
• 5. glycopeptides, , -, -, glycopeptides

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX