Title : Determination of the Glycan Com
position
Abstract :
- CID- MS2 spectra were carefully inspected and manually annotated with respect to the glycan com position
- Major signals in these spectra resulted from consecutive neutral losses (singly and doubly charged species) of the monosaccharides hexose , N-acetylhexosamine and N-acetylneuraminic acid from the intact glycopeptide and most of the time the applied collision energy induced the complete fragmentation of the glycan moiety while leaving the de-glycosylated peptide intact
- These fragment ions along with corresponding oxonium ions, allowed inferring the glycan com position and the putative peptide mass (Fig. 4A)
- Detailed analysis revealed, that exclusively mucin-type core 1 mono- and disialylated O-linked glycopeptides ((di)sialyl-T-antigen) were present
- For the glycan annotation a mass error of ±0.3 Da was accepted
- This parameter was justified as the observed mass errors were about 0.07 Da (median value)
- In total 88 O-glycopeptides were detected and characterized with respect to their glycan com position
- The registered glycopeptides covered an m/z range of 507–945 (average m/z 728) and were either doubly (55 peptides ) or triply charged (33 peptides )
Output (sent_index, trigger,
protein,
sugar,
site):
- 2. de-glycosylated, , -, -, peptide
- 2. glycopeptide, , -, hexose, glycopeptide
- 4. disialylated, , -, -, glycopeptides
- 4. glycopeptides, , -, -, glycopeptides
- 7. O-glycopeptides, , -, -, O-glycopeptides
- 8. charged, , -, -, peptides
- 8. doubly, , -, -, peptides
- 8. glycopeptides, , -, -, glycopeptides
- 8. glycopeptides, , -, -, peptides
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):