PMID: PMC4739677-1-5

 

    Legend: Gene, Sites

Title : Determination of the Glycan Com position

Abstract :
  1. CID- MS2 spectra were carefully inspected and manually annotated with respect to the glycan com position
  2. Major signals in these spectra resulted from consecutive neutral losses (singly and doubly charged species) of the monosaccharides hexose , N-acetylhexosamine and N-acetylneuraminic acid from the intact glycopeptide and most of the time the applied collision energy induced the complete fragmentation of the glycan moiety while leaving the de-glycosylated peptide intact
  3. These fragment ions along with corresponding oxonium ions, allowed inferring the glycan com position and the putative peptide mass (Fig. 4A)
  4. Detailed analysis revealed, that exclusively mucin-type core 1 mono- and disialylated O-linked glycopeptides ((di)sialyl-T-antigen) were present
  5. For the glycan annotation a mass error of ±0.3 Da was accepted
  6. This parameter was justified as the observed mass errors were about 0.07 Da (median value)
  7. In total 88 O-glycopeptides were detected and characterized with respect to their glycan com position
  8. The registered glycopeptides covered an m/z range of 507–945 (average m/z 728) and were either doubly (55 peptides ) or triply charged (33 peptides )
Output (sent_index, trigger, protein, sugar, site):
  • 2. de-glycosylated, , -, -, peptide
  • 2. glycopeptide, , -, hexose, glycopeptide
  • 4. disialylated, , -, -, glycopeptides
  • 4. glycopeptides, , -, -, glycopeptides
  • 7. O-glycopeptides, , -, -, O-glycopeptides
  • 8. charged, , -, -, peptides
  • 8. doubly, , -, -, peptides
  • 8. glycopeptides, , -, -, glycopeptides
  • 8. glycopeptides, , -, -, peptides
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX