Title : Fragment ion spectra of the
Proteinase K generated
plasminogen APPELTPV373 measured with nanoRP-LC-
ESI MSn (positive ion mode, CID and ETD)
Abstract :
- A (top), For the given O-glycopeptide the CID-MS2 spectrum is shown together with its corresponding precursor ion m/z 718.30 [M+3H]3+ (inset)
- The spectrum allows the elucidation of the O-glycan com position (here disialylated T-antigen)
- In addition, also some internal glycopeptide fragments have been detected (e.g. b10\+HexNAc)
- A (bottom): The putative peptide mass (m/z 1205.66 [M+H]+) of the given O-glycopeptide was subjected to CID-MS3 fragmentation
- The peptide was identified by MASCOT search (Score: 16, Uni Prot KB/Swiss- Prot , human)
- B, The O-glycosylation site (here Thr365 ) was pinpointed by means of ETD (Biotools-Score: 150)
- Magnified regions show the isotope pattern of selected peptide fragment ions, confirming the annotation
- In addition to peptide fragment ions also fragment ions derived from the glycan moiety were detected, allowing a verification of the glycan com position
- Furthermore, a neutral loss of an acetyl radical from the intact O-glycopeptide was observed, which is typically seen in ETD spectra of glycopeptides
Output (sent_index, trigger,
protein,
sugar,
site):
- 1. O-glycopeptide, , -, -, O-glycopeptide
- 3. glycopeptide, , -, -, glycopeptide fragments
- 4. O-glycopeptide, , -, -, O-glycopeptide
- 6. B, , -, -, Thr365
- 6. O-glycosylation, , -, -, site
- 9. O-glycopeptide, , -, -, O-glycopeptide
- 9. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):