PMID: PMC4739677-1-fig3

 

    Legend: Gene, Sites

Title : Fragment ion spectra of the Proteinase K generated plasminogen APPELTPV373 measured with nanoRP-LC- ESI MSn (positive ion mode, CID and ETD)

Abstract :
  1. A (top), For the given O-glycopeptide the CID-MS2 spectrum is shown together with its corresponding precursor ion m/z 718.30 [M+3H]3+ (inset)
  2. The spectrum allows the elucidation of the O-glycan com position (here disialylated T-antigen)
  3. In addition, also some internal glycopeptide fragments have been detected (e.g. b10\+HexNAc)
  4. A (bottom): The putative peptide mass (m/z 1205.66 [M+H]+) of the given O-glycopeptide was subjected to CID-MS3 fragmentation
  5. The peptide was identified by MASCOT search (Score: 16, Uni Prot KB/Swiss- Prot , human)
  6. B, The O-glycosylation site (here Thr365 ) was pinpointed by means of ETD (Biotools-Score: 150)
  7. Magnified regions show the isotope pattern of selected peptide fragment ions, confirming the annotation
  8. In addition to peptide fragment ions also fragment ions derived from the glycan moiety were detected, allowing a verification of the glycan com position
  9. Furthermore, a neutral loss of an acetyl radical from the intact O-glycopeptide was observed, which is typically seen in ETD spectra of glycopeptides
Output (sent_index, trigger, protein, sugar, site):
  • 1. O-glycopeptide, , -, -, O-glycopeptide
  • 3. glycopeptide, , -, -, glycopeptide fragments
  • 4. O-glycopeptide, , -, -, O-glycopeptide
  • 6. B, , -, -, Thr365
  • 6. O-glycosylation, , -, -, site
  • 9. O-glycopeptide, , -, -, O-glycopeptide
  • 9. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 6. CID-MS2, -, Thr365

 

 

Protein NCBI ID SENTENCE INDEX