PMID: PMC6145689-1-4

 

    Legend: Gene, Sites

Title : Quantitative comparison of PCa-associated glycan-com positions and lectin blotting analysis

Abstract :
  1. We compared the relative abundance of all intact N-glycopeptides grouped by glycan class and terminal features from PCa and BPH urine
  2. In doing this, no significant differences were observed between PCa and BPH with respect to the overall relative abundance of high mannose , afucosylated/asialylated complex/hybrid (C/H), fucosylated complex/hybrid (C/H\+Fuc) sialylated complex/hybrid (C/H\+NeuAc) and fucosylated/sialylated complex/hybrid (C/H\+Fuc/Sia)
  3. However, increased intensity of Fuc-containing intact desialo-O-glycopeptides was observed (T-test, p < 0.05) (Supplementary Figure 3)
  4. Comparative lectin blotting was also performed using concanavalin A (Con A), Maackia amurensis lectin ( MAL ), wheat germ agglutinin (WGA), Aleuria aurantia lectin (AAL) and Ricinus communis agglutinin (RCA) to assess the level of a range of glycoepitopes in PCa and BPH urine in an orthogonal manner
  5. After protein normalization, significantly increased levels of AAL reactivity were observed for the PCa urinary glycoproteome indicating a higher degree of core fucosylation (Figure 3)
  6. Slightly higher Con A reactivity was also observed in PCa relative to BPH urine, a feature not reflected in the quantitative LC-MS/MS data of high mannose glycopeptides
  7. However, Con A may also cross-react with paucimannosidic and biantennary complex glycoproteins possibly explaining this discrepancy [35]
  8. Based on these observations, we therefore sought to determine whether site-specific glycosylation instead would display a higher potential of discriminating between urine from PCa and BPH patients
Output (sent_index, trigger, protein, sugar, site):
  • 1. N-glycopeptides, , -, -, N-glycopeptides
  • 2. fucosylated, , -, C/H+Fuc, -
  • 2. fucosylated/sialylated, , -, C/H+Fuc/Sia, -
  • 2. sialylated, , -, C/H+NeuAc, -
  • 3. desialo-O-glycopeptides, , -, -, desialo-O-glycopeptides
  • 6. glycopeptides, , -, -, glycopeptides
  • 7. glycoproteins, , glycoproteins, -, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX