PMID: PMC6145689-1-fig1

 

    Legend: Gene, Sites

Title : TMT label-assisted quantitative glycoproteomics of urinary glycoprotein from PCa and BPH patients (A) The serum PSA levels of PCa (n = 6) and BPH (n = 6) patients showed no statistical difference (p > 0.05, unpaired two tailed t-test)

Abstract :
  1. (B) Overview of the quantitative glycoproteomics workflow employed in this study
  2. Peptides were generated from extracted urinary proteins from each patient, labelled with isobaric TMT tags and mixed 1:1 (w:w)
  3. The glycopeptides were selectively enriched using either TiO2 or HILIC SPE and the resulting fractions were either analyzed directly or after simultaneous endo- and exoglycosidase treatment by LC-MS/MS
  4. Former N-glycosylated peptides generated by PNGase F (referred to as de-N-glycosylated peptides ) and desialo-O-glycosylated peptides generated by sialidase and non-modified peptides were pre-fractionated using off-line HILIC HPLC; the resulting fractions were analyzed by LC-MS/MS
  5. Different information was obtained from the analysis of intact glycopeptides (red), de-N-glycopeptides (green) and non-modified peptides (blue)
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycoprotein, , glycoprotein, -, -
  • 3. glycopeptides, , -, -, glycopeptides
  • 4. N-glycosylated, , -, -, peptides
  • 4. de-N-glycosylated, , -, -, peptides
  • 4. desialo-O-glycosylated, , -, -, peptides
  • 5. de-N-glycopeptides, , -, -, de-N-glycopeptides
  • 5. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX