Title :
TMT label-assisted quantitative glycoproteomics of urinary
glycoprotein from PCa and BPH patients (A) The serum
PSA levels of PCa (n = 6) and BPH (n = 6) patients showed no statistical difference (p > 0.05, unpaired two tailed t-test)
Abstract :
- (B) Overview of the quantitative glycoproteomics workflow employed in this study
- Peptides were generated from extracted urinary proteins from each patient, labelled with isobaric TMT tags and mixed 1:1 (w:w)
- The glycopeptides were selectively enriched using either TiO2 or HILIC SPE and the resulting fractions were either analyzed directly or after simultaneous endo- and exoglycosidase treatment by LC-MS/MS
- Former N-glycosylated peptides generated by PNGase F (referred to as de-N-glycosylated peptides ) and desialo-O-glycosylated peptides generated by sialidase and non-modified peptides were pre-fractionated using off-line HILIC HPLC; the resulting fractions were analyzed by LC-MS/MS
- Different information was obtained from the analysis of intact glycopeptides (red), de-N-glycopeptides (green) and non-modified peptides (blue)
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoprotein, , glycoprotein, -, -
- 3. glycopeptides, , -, -, glycopeptides
- 4. N-glycosylated, , -, -, peptides
- 4. de-N-glycosylated, , -, -, peptides
- 4. desialo-O-glycosylated, , -, -, peptides
- 5. de-N-glycopeptides, , -, -, de-N-glycopeptides
- 5. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):