Title : Mapping of O‐linked glycoproteome Workflow to map site‐specific O‐linked glycoproteome in human samples
Abstract :
- Distribution of EXoO identified peptides in different samples
- Unsupervised hierarchical clustering of samples and PSM number of peptides to show that EXoO identified peptides exhibited different distribution and relative abundance in samples
- Euclidean distance and city block distance were used for clustering of samples and peptides, respectively
- Analysis of amino acid sequence surrounding O‐linked glycosylation sites
- Cellular component analysis of O‐linked glycoproteins
- Landscape distribution of the O‐linked glycosylation sites and its frequency in proteins
- Relative position was calculated using amino acid position of the sites to divide total number of amino acids of the protein and time a hundred to show in percentage
- Lower panel: Relative position of the sites in proteins
- Proteins with positions close to protein N‐termini were ranked to the top
- Middle panel: Relative position of sites in five proteins with the highest number of sites
- Protein with higher number of sites was ranked to the top
- Upper panel: Frequency of the sites in proteins
- The y‐axis of upper panel shows the number of sites at the relative position of proteins
Output (sent_index, trigger,
protein,
sugar,
site):
- 4. glycosylation, , -, -, sites
- 5. glycoproteins, , glycoproteins, -, -
- 6. glycosylation, , -, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 10. proteins, position
- 13. proteins, position
- 8. proteins, position
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):