Title : Comparative analysis of the
O‐linked glycoproteome of normal and tumor‐derived kidney tissue Data processing steps in label‐free quantification to identify differential
glycoproteins between tumor and normal kidney tissues
Abstract :
- Volcano plot of differentially expressed O‐linked glycoproteins between tumor and normal tissues
- A total of 592 glycoproteins are plotted in the Volcano plot according to their fold change (log2) and number of differential PSM between cancer and normal (log10)
- Red and green dots indicate significantly upregulated and downregulated proteins , respectively
- Extensive addition of O‐linked glycans at sites covering versican core protein ( VCAN ) (upper panel) and aggrecan core protein ( ACAN ) (lower panel)
- Fold change for specific site between tumor and normal was showed as connected dots in red
- Sites only detected in tumor or normal were assigned two‐ or 0.5‐fold change, respectively
- Red asterisks indicate sites with the highest PSM number divergent between tumor and normal tissues
- The 56 O‐linked glycoproteins with significant change between tumor and normal
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoproteins, , glycoproteins, -, -
- 1. glycoproteins, , glycoproteins, -, -
- 2. glycoproteins, , glycoproteins, -, -
- 8. glycoproteins, , glycoproteins, -, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):