Title : Schematic representationand validation of the experimental workflowfor the identification of
MGL ligands in Jurkat cells
Abstract :
- (A) Pull-downworkflow to capture MGL-binding proteins
- As negative control, EDTAwas added to the sample prior to incubation with MGL-Fc to preventthe binding to MGL
- (B) Western blot analysis using monoclonal anti-CD43staining of the unbound fraction and captured proteins (elution) fromthe MGL-Fc pull-down assay in the absence (−) and presence (+,negative control) of EDTA (100 mM)
- Fc: Fragment crystallizable region ; Prot G: protein G; TCL : total cell lysate
Output (sent_index, trigger,
protein,
sugar,
site):
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):