PMID: PMC6399673-1-fig1

 

    Legend: Gene, Sites

Title : Schematic representationand validation of the experimental workflowfor the identification of MGL ligands in Jurkat cells

Abstract :
  1. (A) Pull-downworkflow to capture MGL-binding proteins
  2. As negative control, EDTAwas added to the sample prior to incubation with MGL-Fc to preventthe binding to MGL
  3. (B) Western blot analysis using monoclonal anti-CD43staining of the unbound fraction and captured proteins (elution) fromthe MGL-Fc pull-down assay in the absence (−) and presence (+,negative control) of EDTA (100 mM)
  4. Fc: Fragment crystallizable region ; Prot G: protein G; TCL : total cell lysate
Output (sent_index, trigger, protein, sugar, site):
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX