PMID: PMC6428843-1-fig1

 

    Legend: Gene, Sites

Title : Identifying intact glycopeptides with AI-ETD

Abstract :
  1. a Annotated single AI-ETD spectrum (i.e., no averaging) of N-glycopeptide TN*SSFIQGFVDHVKEDcDR modified with a high mannose-type glycan [HexNAc(2)Hex(9)]
  2. The red asparagine indicates the site of glycosylation, and the lowercase cysteine indicates carbamidomethylation
  3. Green fragments are products from peptide backbone cleavage, triply charged Y-ions are annotated along the top, and B-ions include only glycan moieties
  4. Blue asterisks (*) denote doubly and quadruply charged Y-ions (from 1700 to 2000 and 750 to 1000 Th, respectively), each which differ by one hexose residue
  5. Peptide fragments retain the glycan modification unless denoted by a β€œ~”
  6. b Distribution of percent peptide backbone coverage and glycan coverage seen in AI-ETD spectra
  7. Median and quartile values are provided by the center line and box boundaries, respectively
  8. Whiskers show 10th and 90th percentiles, and the small square indicates the average
  9. c Average percent of explained ion current in product ions in AI-ETD spectra from peptide backbone cleavage fragments , Y-ions (i.e., intact peptide sequence with fragments of the glycan moiety), and B-ions/oxonium ions
  10. d Distribution of precursor ion charge states successfully identified in the 24,099 glyco PSMs from this study, given as a percentage of the total
  11. e Comparison of recent large-scale N-glycopeptide studies showing the number of unique N-glycopeptides (left axis, dark blue line) and unique N-glycosites (right axis, light blue bars) identified
  12. Asterisks (*) by the study name indicate that mouse brain was the system investigate
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycopeptides, , -, -, glycopeptides
  • 1. N-glycopeptide, , -, -, N-glycopeptide
  • 11. N-glycopeptide, , -, -, N-glycopeptide
  • 11. N-glycopeptides, , -, -, N-glycopeptides
  • 11. N-glycosites, , -, -, N-glycosites
  • 2. glycosylation, , -, -, site
Output(Part-Of) (sent_index, protein, site):
  • 3. Green, fragments
  • 5. Peptide, fragments
  • 9. -, fragments
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX