PMID: PMC7108604-1-5

 

    Legend: Gene, Sites

Title : Distribution of sialic acids and junctional cell adhesion molecules in endothelial cells in situ and in culture

Abstract :
  1. Based on the glycosylation data described above, the localization of sialic acids and Ca2 +-dependent VE-cadherin was studied in endothelial cells of the human umbilical vein and arteries in situ as well as in culture (Figures 6, 7)
  2. As a control, Ca2 +-independent PECAM-1 was also localized (Figure 8)
  3. Digoxigenin labeled MAA- and SNA-lectins, used together or individually (MAA binds to α2,3-linked and SNA binds to α2,6-linked sialic acids), stained the intercellular junctions as well as surface proteins of endothelial cells in situ (Figure 6) and in culture (Figures 7, 8)
  4. Sialic acids, found at interendothelial junctions, largely colocalized with VE-cadherin in a not interrupted continuous pattern in endothelial cells of human umbilical vein and arteries in situ (Figure 6) and in culture (Figure 7)
  5. In addition, at overlapping junctional areas of adjacent highly confluent endothelial cells a netlike distribution of VE-cadherin was observed (Figure 7A,B,D,E) that, again, largely colocalized with MAA/SNA-staining (Figure 7B1,D1,E1)
  6. This netlike structure has not been described before and represents a highly ordered VE-cadherin organization only observable in confluent endothelial cell cultures
  7. Therefore, we termed it VE-cadherin superstructure
  8. Double labeling of VE-cadherin and catenins also displayed a codistribution of this highly organized structure (not shown)
  9. PECAM-1 , labeled with a monoclonal antibody, partially colo-calized with MAA/SNA but to a lesser extent than VE-cadherin (Figure 8C,D)
  10. This was further confirmed by serial optical z-sections using confocal laser microscopy
  11. Whereas sialic acids and VE-cadherin were primarily localized within the apical area of intercellular junctions, PECAM-1 appears to reside predominantly at the basal side (data not shown)
Output (sent_index, trigger, protein, sugar, site):
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

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