PMID: PMC7108604-1-6

 

    Legend: Gene, Sites

Title : Effect of extracellular Ca2 +-depletion on sialic acid distribution

Abstract :

1. To investigate whether junctional staining with MAA/SNA was mediated by sialic acids linked to Ca2 +-dependent molecules, Ca2 +-depletion experiments were performed
2. Incubation of cell monolayers with 3 mM EGTA for 30 min (leading to an extracellular Ca2 +-concentration of <10–7 M) caused a complete absence of VE-cadherin and an almost complete disappearance of the MAA/SNA staining from interendothelial junctions (Figure 7C,C1) whereas PECAM-1 remained unchanged (Figure 8A,B)
3. A very weak junctional staining of sialic acids, leftover after Ca2 +-depletion (Figure 7C1, 8A), might be caused by the remaining PECAM-1 and/or additional Ca2 +-independent components
4. After recalcification (1.8 mM of extracellular Ca2 +), VE-cadherin and MAA/SNA staining completely reappeared at the junctions within 30 min (Figure 7D,D1,E,E1)
5. These findings are in line with previous observations showing a reappearance of VE-cadherin within 5 min after recalcification (Ayalon et al., 1994; Dejana ; Schnittler )
6. They further indicate that sialic acids located at the interendothelial junctions are predominantly bound to Ca2 +-dependent molecules, presumably VE-cadherin
7. Preabsorption of MAA/SNA lectins with sialylated pig stomach type III mucin completely abolished the lectin staining in all experimental approaches

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX