PMID: PMC7108604-1-fig2

 

    Legend: Gene, Sites

Title : Fractionation of PNGase F-released oligosaccharides from human VE-cadherin by anion-exchange HPLC

Abstract :
  1. (A), oligosaccharide alditols obtained after treatment of endo H-resistant glycopeptides with PNGase F , RP-HPLC and reduction were subjected to anion-exchange HPLC using a Mikropak AX-10 column (4.6 × 250 mm) and a linear gradient of 0–300 mM potassium phosphate, pH 4 .4, within 60 min
  2. Fractions (400 µl) were collected at 1 ml/min and monitored for radioactivity
  3. Fractions (F0–F3) were pooled as indicated by brackets
  4. (B–D) Rechromatography of F1- (B), F2- (C), and F3- (D) derived glycans after treatment with α2,3-specific sialidase from Newcastle disease virus under similar conditions
  5. Numbers (0–4) with arrows indicate the elution volumes of standard oligosaccharides with 0–4 sialic acid residues
  6. * in (C) and (D), released sialic acid
Output (sent_index, trigger, protein, sugar, site):
  • 1. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX