Title : Fractionation of
PNGase F-released oligosaccharides from human
VE-cadherin by anion-exchange HPLC
Abstract :
- (A), oligosaccharide alditols obtained after treatment of endo H-resistant glycopeptides with PNGase F , RP-HPLC and reduction were subjected to anion-exchange HPLC using a Mikropak AX-10 column (4.6 × 250 mm) and a linear gradient of 0–300 mM potassium phosphate, pH 4 .4, within 60 min
- Fractions (400 µl) were collected at 1 ml/min and monitored for radioactivity
- Fractions (F0–F3) were pooled as indicated by brackets
- (B–D) Rechromatography of F1- (B), F2- (C), and F3- (D) derived glycans after treatment with α2,3-specific sialidase from Newcastle disease virus under similar conditions
- Numbers (0–4) with arrows indicate the elution volumes of standard oligosaccharides with 0–4 sialic acid residues
- * in (C) and (D), released sialic acid
Output (sent_index, trigger,
protein,
sugar,
site):
- 1. glycopeptides, , -, -, glycopeptides
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):