PMID: PMC7108604-1-fig3

 

    Legend: Gene, Sites

Title : Chromatographic profile of desialylated endo H-sensitive glycans from human VE-cadherin

Abstract :

1. (A) Oligosaccharide alditols obtained after treatment of proteolytic glycopeptides with endo H, RP-HPLC, reduction and enzymatic desialylation were separated by HPAEC on a CarboPak PA-100 column (4 × 250 mm) using a gradient of 10–30 mM sodium acetate in 80 mM NaOH within 70 min
2. Fractions (380 µl) were collected at 1 ml/min and monitored for radioactivity
3. (B) Desialylated endo H-sensitive glycans after treatment with α-mannosidase ; (C) desialylated endo H-sensitive glycans after treatment with β-galactosidase from D.pneumoniae
4. Numbers (OM5-9) with arrows indicate the elution volumes of oligomannosidic oligosaccharide standard alditols Man5-9GlcNAcOH; MIII, MIV, elution volumes of hybrid-type oligosaccharide standard alditols Galβ4GlcNAcβ2Manα3[Manα3Manα6]Manβ4GlcNAcOH and Galβ4GlcNAcβ2Manα3[Manα3(Manα6)Manα6]Manβ4GlcNAcOH, respectively; I, II, elution volumes of Manβ4GlcNAcOH and Galβ4GlcNAcβ2Manα3Manβ4GlcNAcOH.
5. * in (C), unidentified product

Output (sent_index, trigger, protein, sugar, site):

• 1. desialylation, , -, -, glycopeptides
• 1. glycopeptides, , -, -, glycopeptides

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX